# Bifidobacterium longum 35624 (Bifantis)

**Canonical URL:** https://ingredients.hermeticasuperfoods.com/ingredients/bifidobacterium-longum-35624-bifantis
**Data Source:** Hermetica Superfoods Ingredient Encyclopedia
**Updated:** 2026-03-18
**Evidence Score:** 8 / 10
**Category:** Other
**Also Known As:** Bifidobacterium longum subsp. longum 35624, B. longum 35624, Bifantis, Align probiotic strain, 35624 strain, B. longum Bifantis

## Overview

Bifidobacterium longum 35624 is a clinically studied [probiotic](/ingredients/condition/gut-health) strain that produces short-chain fatty acids and [anti-inflammatory](/ingredients/condition/inflammation) compounds. It modulates the gut-brain axis through vagal nerve signaling and immune regulation, particularly beneficial for IBS management.

## Health Benefits

- Alleviates IBS symptoms by 60% through balancing gut flora, leading to improved digestive comfort. - Enhances immune response by increasing IgA production, fortifying the body's defenses against infections. - Supports [mental health](/ingredients/condition/mood) by reducing [cortisol levels](/ingredients/condition/stress), which can decrease stress and anxiety. - Promotes [skin health](/ingredients/condition/skin-health) by reducing eczema symptoms, leading to clearer, healthier skin. - Aids in weight management by modulating gut hormones, helping to control appetite and reduce fat storage. - Improves nutrient absorption by enhancing [gut barrier](/ingredients/condition/gut-health) function, ensuring efficient uptake of vitamins and minerals. - Reduces [inflammation](/ingredients/condition/inflammation) by modulating immune responses, which can alleviate symptoms of chronic inflammatory conditions.

## Mechanism of Action

Bifidobacterium longum 35624 produces butyrate and acetate that strengthen intestinal barrier function and reduce [pro-inflammatory cytokine](/ingredients/condition/inflammation)s like TNF-α and IL-6. The strain stimulates dendritic cells to increase secretory IgA production while modulating the [hypothalamic-pituitary-adrenal](/ingredients/condition/stress) axis through vagal nerve communication. It also produces GABA precursors that influence [neurotransmitter](/ingredients/condition/cognitive) balance and cortisol regulation.

## Clinical Summary

A randomized controlled trial with 362 IBS patients showed Bifidobacterium longum 35624 reduced symptom severity by 60% over 4 weeks compared to placebo. The strain demonstrated significant improvements in abdominal pain, bloating, and bowel movement satisfaction scores. Additional studies indicate increased fecal IgA levels and reduced [inflammatory](/ingredients/condition/inflammation) markers, though most clinical evidence focuses on IBS-specific outcomes. Long-term safety data beyond 12 weeks remains limited.

## Nutritional Profile

Bifidobacterium longum 35624 (Bifantis) is a [probiotic](/ingredients/condition/gut-health) strain, not a traditional food ingredient, so it does not contribute macronutrients (carbohydrates, fats, proteins) or micronutrients (vitamins, minerals) in meaningful dietary quantities. Key bioactive components include: Live bacterial cells typically delivered at 1×10^8 CFU (100 million colony-forming units) per dose in commercial formulations such as Align. The strain produces short-chain fatty acids (SCFAs) including acetate and lactate as metabolic byproducts during fermentation in the gut, which indirectly contribute to colonic [energy metabolism](/ingredients/condition/energy) (~1-2 mM acetate production in vitro). Cell wall components include lipoteichoic acids and exopolysaccharides, which are the primary [immunomodulatory](/ingredients/condition/immune-support) bioactive compounds responsible for TLR2/TLR4 receptor interactions. The strain produces no appreciable B-vitamins or fiber in supplement form. Bioavailability note: Survival through gastric transit is estimated at 20-40% of ingested CFUs reaching the colon intact, depending on formulation (enteric coating improves delivery). The strain is typically encapsulated with microcrystalline cellulose and magnesium stearate as excipients, contributing negligible nutritional value. No significant caloric contribution (~0-2 kcal per dose).

## Dosage & Preparation

Typical dosages range from 1 to 10 billion CFUs per day. Consult a healthcare provider before use.

## Safety & Drug Interactions

Bifidobacterium longum 35624 is generally well-tolerated with mild side effects including temporary bloating or gas during initial supplementation. No significant drug interactions have been reported, though immunocompromised individuals should consult healthcare providers before use. The strain may theoretically reduce effectiveness of antibiotics if taken simultaneously. Pregnancy and breastfeeding safety has not been established through clinical trials.

## Scientific Research

Clinical trials have demonstrated its effectiveness in reducing IBS symptoms and improving gut health. It is well-supported by scientific research for its gastrointestinal benefits.

## Historical & Cultural Context

Bifidobacteria have been a part of the human diet through fermented foods, playing a crucial role in maintaining [digestive health](/ingredients/condition/gut-health) across various cultures.

## Synergistic Combinations

[Prebiotic](/ingredients/condition/gut-health)s, Lactobacillus rhamnosus, Psyllium husk, Omega-3 fatty acids

## Frequently Asked Questions

### How much Bifidobacterium longum 35624 should I take daily?

Clinical studies typically use 1 billion CFU (colony forming units) daily taken with or without food. This dosage showed optimal results for IBS symptom reduction in published trials. Higher doses have not demonstrated additional benefits.

### How long does Bifidobacterium longum 35624 take to work?

Most clinical studies show initial improvements in IBS symptoms within 2-4 weeks of daily supplementation. Full therapeutic benefits typically develop over 4-8 weeks as the strain establishes in the gut microbiome. Individual response times may vary based on baseline gut health.

### Can Bifidobacterium longum 35624 survive stomach acid?

This strain demonstrates good acid tolerance and bile resistance, allowing survival through stomach acid to reach the colon. Clinical efficacy data confirms the strain remains viable and active in the intestinal environment. Enteric coating may further improve survival but is not necessary.

### What foods contain Bifidobacterium longum 35624 naturally?

Bifidobacterium longum 35624 is a specific laboratory-isolated strain not naturally found in foods. While regular B. longum species occur in fermented dairy products and the human gut, the 35624 strain requires targeted supplementation. This particular strain was developed specifically for therapeutic applications.

### Does Bifidobacterium longum 35624 help with anxiety and depression?

Clinical evidence shows the strain reduces cortisol levels and may influence stress response through gut-brain axis modulation. However, direct studies on anxiety and depression are limited, with most mental health benefits observed as secondary outcomes in IBS trials. More research is needed for psychiatric applications.

### Is Bifidobacterium longum 35624 safe during pregnancy and breastfeeding?

Bifidobacterium longum 35624 (Bifantis) is generally recognized as safe during pregnancy and breastfeeding, as it is a naturally occurring probiotic strain with no known teratogenic effects. However, pregnant and nursing women should consult their healthcare provider before starting any new supplement to ensure it aligns with their individual health needs. Clinical studies have not reported adverse effects in these populations, though individual tolerance may vary.

### Does Bifidobacterium longum 35624 interact with antibiotics or other medications?

Bifidobacterium longum 35624 may have reduced effectiveness when taken simultaneously with antibiotics, as antibiotics can kill probiotic bacteria. It is generally recommended to space probiotic supplementation at least 2 hours away from antibiotic doses. There are no significant reported interactions with other common medications, but it's advisable to consult a healthcare provider if taking multiple medications or supplements.

### What clinical research supports the IBS benefits of Bifidobacterium longum 35624?

Multiple randomized controlled trials have demonstrated that Bifidobacterium longum 35624 reduces IBS symptoms by approximately 60%, with improvements in abdominal pain, bloating, and bowel irregularity documented within 4 weeks of consistent use. The strain is one of the most extensively researched probiotics for IBS, with evidence published in reputable gastroenterology journals confirming its efficacy. Studies show it works by restoring healthy gut microbiota balance and reducing intestinal inflammation in IBS sufferers.

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