# Lactococcus lactis subsp. cremoris MG1363 **Canonical URL:** https://ingredients.hermeticasuperfoods.com/ingredients/lactococcus-lactis-subsp-cremoris-mg1363 **Data Source:** Hermetica Superfoods Ingredient Encyclopedia **Updated:** 2026-03-29 **Evidence Score:** 2 / 10 **Category:** Fermented/Probiotic **Also Known As:** MG1363, L. lactis subsp. cremoris MG1363, Lactococcus lactis MG1363, plasmid-free MG1363, NCDO712 plasmid-cured derivative, Ll. lactis subsp. cremoris MG1363 ## Overview Lactococcus lactis subsp. cremoris MG1363 is a plasmid-free, fully sequenced laboratory reference strain derived from the NCDO712 lineage, used extensively in bacterial genetics research. It serves as a model organism for studying lactic acid bacteria gene expression, bacteriocin biosynthesis pathways, and horizontal gene transfer mechanisms rather than as a consumable supplement. ## Health Benefits • No clinical health benefits have been studied or documented for this laboratory research strain • The strain is used exclusively for genetic and genomic research rather than as a [probiotic](/ingredients/condition/gut-health) supplement • Parent Lactococcus lactis strains are used in food fermentation but not for therapeutic purposes • No evidence exists for immune, digestive, or other health effects in humans • Research focuses on its use as a model organism for protein expression systems rather than health applications ## Mechanism of Action MG1363 is a plasmid-cured derivative of NCDO712 used to study the genetic machinery of lactic acid bacteria, particularly nisin biosynthesis gene clusters and the NisRK two-component regulatory system. Researchers exploit its well-characterized chromosome to investigate PTS (phosphoenolpyruvate-dependent phosphotransferase system) sugar transport enzymes and homologous recombination pathways. Its lack of endogenous plasmids makes it an ideal host for introducing recombinant DNA constructs to study promoter activity and protein secretion via the Sec and Tat translocon pathways. ## Clinical Summary No human clinical trials have been conducted using MG1363 as a therapeutic or [probiotic](/ingredients/condition/gut-health) intervention, as this strain is designated exclusively for in vitro and microbiology laboratory use. All published research involves bench-scale genetic manipulation studies, fermentation biochemistry experiments, and bacterial genomics analyses with no human subjects. The complete genome sequence, published by Wegmann et al. (2007) in the Journal of Bacteriology, established MG1363 as the definitive reference genome for Lactococcus lactis subsp. cremoris, enabling comparative genomic studies. Evidence for any health benefit in humans is entirely absent, and the strain should not be conflated with probiotic Lactococcus lactis strains evaluated in food science contexts. ## Nutritional Profile As a laboratory research strain rather than a food or supplement ingredient, MG1363 lacks a meaningful nutritional profile for human consumption contexts. At the cellular composition level, Lactococcus lactis bacteria generally consist of approximately 50-60% protein by dry weight, 15-25% nucleic acids, 10-15% lipids (primarily membrane phospholipids such as phosphatidylglycerol and cardiolipin), and 5-10% carbohydrates in the form of cell wall polysaccharides and peptidoglycans. MG1363 is a plasmid-cured derivative of L. lactis subsp. cremoris 712, meaning it has been stripped of naturally occurring plasmids, making it genetically simpler but not representative of strains used in food production. It does not produce nisin (an [antimicrobial](/ingredients/condition/immune-support) peptide produced by some L. lactis strains) due to its plasmid-free status. The strain can produce small amounts of B vitamins including riboflavin (B2) and folate as metabolic byproducts during growth, consistent with L. lactis species generally, but no quantified data exists for MG1363 specifically in a human supplementation context. Bioavailability of any cellular components is effectively irrelevant given this strain is not intended for ingestion. ## Dosage & Preparation No clinically studied dosage ranges are available as no human clinical studies exist for this strain. MG1363 is not formulated as a [probiotic](/ingredients/condition/gut-health) extract, powder, or standardized product for clinical use but is cultured exclusively for laboratory applications. Consult a healthcare provider before starting any new supplement. ## Safety & Drug Interactions MG1363 is not formulated as a supplement and has no established safety profile, dosing guidelines, or documented human exposure data in a therapeutic context. As a non-pathogenic lactic acid bacteria strain classified under BSL-1, it is not considered hazardous in laboratory settings, but its safety as an ingestible agent has never been evaluated in clinical or toxicological studies. No drug interactions, contraindications, or pregnancy safety assessments exist because this strain has not progressed beyond laboratory research. Individuals should not attempt to self-administer research-grade bacterial strains, as sterility, viability, and contaminant profiles of lab cultures do not meet standards for human consumption. ## Scientific Research No human clinical trials, RCTs, or meta-analyses were identified for L. lactis subsp. cremoris MG1363 in the available research. Published studies such as PMID 7751295 focus exclusively on genetic mapping and basic laboratory research rather than clinical applications. This strain serves primarily as a laboratory model for genetic, genomic, and physiological studies in lactic acid bacteria, particularly for protein expression via the NICE system. ## Historical & Cultural Context No historical traditional medicine uses were identified for MG1363 specifically, as it is a modern plasmid-cured laboratory derivative created for research purposes. While parent Lactococcus lactis strains have been used in food fermentation such as cheese-making for centuries in European dairy traditions, they were not used as medicine. ## Synergistic Combinations Because MG1363 is strictly a genetic research tool and not a consumable probiotic or food ingredient, no evidence-based synergy stack exists for human health applications. If discussing its parent species L. lactis in legitimate fermented food contexts, the species demonstrates synergy with Streptococcus thermophilus through complementary lactase activity and protocooperation during dairy fermentation, with Leuconostoc mesenteroides for enhanced diacetyl and flavor compound production via shared citrate [metabolism](/ingredients/condition/weight-management) pathways, and with [prebiotic](/ingredients/condition/gut-health) galactooligosaccharides (GOS) which support lactococcal colonization through preferential fermentation. Any synergy claims specifically for MG1363 in a health supplement context would be scientifically unsupported, as this strain's documented interactions are limited to laboratory co-culture experiments studying gene transfer and metabolic pathway engineering rather than human physiological outcomes. ## Frequently Asked Questions ### Is Lactococcus lactis subsp. cremoris MG1363 a probiotic supplement? No, MG1363 is not a probiotic supplement. It is a plasmid-free laboratory reference strain used exclusively for genetic and genomic research, and it has never been formulated or clinically evaluated for human health use. Probiotic Lactococcus lactis products use entirely different, commercially validated strains. ### What is Lactococcus lactis subsp. cremoris MG1363 used for in research? MG1363 is used as a model organism to study gene expression, bacteriocin biosynthesis (particularly the nisin gene cluster regulated by the NisRK two-component system), and plasmid biology in lactic acid bacteria. Its fully sequenced, plasmid-free chromosome, published by Wegmann et al. in 2007, makes it the standard reference genome for the cremoris subspecies. It is also widely used as a host for recombinant protein expression and secretion studies. ### What is the difference between MG1363 and probiotic Lactococcus lactis strains? MG1363 is a plasmid-cured, genetically manipulated derivative of NCDO712 bred specifically for laboratory tractability, lacking the native plasmids that confer traits like lactose metabolism and protease activity found in food-grade strains. Probiotic or food fermentation strains such as Lactococcus lactis subsp. lactis IL1403 or various dairy starter cultures retain functional plasmids and have undergone safety assessments. MG1363 has no documented probiotic properties and is not produced to food-grade standards. ### Does Lactococcus lactis subsp. cremoris MG1363 produce nisin? MG1363 itself does not naturally produce nisin, as it lacks the nisABTCIPRKFEG gene cluster in its wild-type form. However, researchers frequently introduce nisin biosynthesis plasmids into MG1363 to study nisin regulation via the NisRK sensor kinase and response regulator system. This makes MG1363 a key experimental tool for understanding how this antimicrobial peptide is biosynthesized and regulated, though not a natural nisin producer. ### Can you buy Lactococcus lactis subsp. cremoris MG1363 as a supplement? MG1363 is not commercially available as a supplement and is only distributed through microbiology culture collections such as the ATCC or DSMZ for laboratory research purposes. No supplement manufacturer produces or sells this strain for human consumption, and regulatory agencies have not reviewed it for use as a dietary supplement. Consumers seeking probiotic benefits from lactic acid bacteria should look for commercially validated strains with documented clinical evidence. ### Is Lactococcus lactis subsp. cremoris MG1363 safe for human consumption? MG1363 is a laboratory research strain not intended for human consumption and has not undergone safety testing in humans. This strain is exclusively used in controlled research environments for genetic and genomic studies, not as a food ingredient or supplement. If you are considering any Lactococcus lactis product, verify it is a food-grade or clinically tested probiotic strain rather than a research isolate. ### What scientific research exists on Lactococcus lactis subsp. cremoris MG1363 health effects? No clinical research has documented health benefits of MG1363 in humans, as this strain is not designed for therapeutic use. Available research focuses exclusively on genetic engineering, protein expression, and genomic characterization in laboratory settings. Any health claims about this specific strain should be treated with significant skepticism, as evidence is limited to in vitro and microbial studies only. ### How is Lactococcus lactis subsp. cremoris MG1363 different from food-grade Lactococcus lactis strains? MG1363 is a genetically characterized research strain used for experimental microbiology, while food-grade Lactococcus lactis strains are used in dairy fermentation and have safety histories in food production. MG1363 lacks the safety and efficacy data required for use as a food additive or probiotic supplement. Food-grade strains may have traditional use in cheese and yogurt production, but neither category has established therapeutic probiotic benefits in humans. --- *Source: Hermetica Superfoods Ingredient Encyclopedia — https://ingredients.hermeticasuperfoods.com* *License: CC BY-NC-SA 4.0 — Attribution required. Commercial use: admin@hermeticasuperfoods.com*