# Indigo Berry **Canonical URL:** https://ingredients.hermeticasuperfoods.com/ingredients/indigo-berry **Data Source:** Hermetica Superfoods Ingredient Encyclopedia **Updated:** 2026-03-15 **Evidence Score:** 6 / 10 **Category:** Berry **Also Known As:** Indigofera tinctoria fruit, Indigo plant berry, True indigo berry, Anil berry ## Overview Indigo berry, the fruit of Polygonum tinctorium (Japanese indigo), is rich in the iridoid glycoside aucubin and diverse polyphenols—including flavonoids, phenolic acids, and anthocyanins—that confer documented [antioxidant](/ingredients/condition/antioxidant), anticancer, and [anti-inflammatory](/ingredients/condition/inflammation) properties. Duan et al. (2019, PMID 31467086) demonstrated that aucubin isolated from indigo fruits protected mice against LPS-induced cardiac dysfunction by suppressing NF-κB signaling, while Jang et al. (2012, PMID 22644642) identified potent antioxidant and anticancer polyphenols in Polygonum tinctorium seeds and leaves. ## Health Benefits - **May possess mild**: [antimicrobial](/ingredients/condition/immune-support) properties, primarily identified in seed extracts. - **May exhibit preliminary**: [anti-inflammatory](/ingredients/condition/inflammation) effects based on phytochemical analysis. - **Primarily studied for**: its phytochemical presence rather than direct nutritional benefits as a consumable fruit. ## Mechanism of Action Aucubin, the principal bioactive iridoid glycoside in Polygonum tinctorium fruits, exerts cardioprotective and anti-inflammatory effects by directly inhibiting IκB kinase (IKK) phosphorylation, thereby preventing proteasomal degradation of the inhibitory protein IκBα and blocking nuclear translocation of the pro-inflammatory transcription factor NF-κB (PMID 31467086). This suppression of the IKK/IκBα/NF-κB axis reduces downstream expression of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and other [pro-inflammatory cytokine](/ingredients/condition/inflammation)s implicated in endotoxemia-associated cardiac injury. Additionally, polyphenolic constituents—including flavonoid glycosides and phenolic acids identified in Polygonum tinctorium (PMID 22644642)—scavenge [reactive oxygen species](/ingredients/condition/antioxidant) (ROS) and chelate transition metal ions, protecting cellular lipids, proteins, and DNA from oxidative damage. Anthocyanins present in the fruit further contribute to redox modulation by upregulating endogenous antioxidant enzymes such as superoxide dismutase (SOD) and catalase through Nrf2-ARE pathway activation. ## Clinical Summary Scientific studies on Indigofera species focus predominantly on phytochemical profiling and in vitro [antimicrobial](/ingredients/condition/immune-support) testing of seed extracts rather than clinical trials. No randomized controlled trials have been conducted specifically on Indigo Berry fruit consumption. Current evidence is limited to laboratory analysis of bioactive compounds and preliminary antimicrobial screening studies. The fruit's purported health benefits lack robust clinical validation. ## Nutritional Profile - Low levels of Indican (a precursor to indigo) and small amounts of flavonoids. - Contains alkaloids, primarily identified in seeds, with potential [antimicrobial](/ingredients/condition/immune-support) properties. - Not considered nutritionally dense and lacks the phytochemical potency of the Indigo plant's leaves. ## Dosage & Preparation - Traditional Use: Rarely used in traditional formulations; the seeds may be ground for topical applications in some folk medicine. - Internal Consumption: Not traditionally consumed as food. - Dosage: No established dosage due to limited internal use and lack of traditional food application. ## Safety & Drug Interactions No clinical toxicology studies specific to Polygonum tinctorium fruit consumption in humans have been published to date; safety data are currently extrapolated from traditional use in East Asian medicine and limited preclinical murine models (PMID 31467086). Because aucubin and related iridoid glycosides may modulate NF-κB signaling and inflammatory cytokine release, theoretical interactions exist with immunosuppressant drugs (e.g., methotrexate, cyclosporine) and non-steroidal [anti-inflammatory](/ingredients/condition/inflammation) agents (NSAIDs), though no specific CYP450 enzyme inhibition data for indigo berry compounds have been reported. Individuals on anticoagulant therapy should exercise caution, as polyphenol-rich berry extracts may exhibit mild antiplatelet activity. Pregnant or nursing women and individuals with hepatic impairment should consult a healthcare provider before consuming indigo berry preparations, given the absence of formal safety evaluations in these populations. ## Scientific Research Jang et al. (2012) published in Applied Biochemistry and Biotechnology (PMID 22644642) performed comprehensive phytochemical profiling of Polygonum tinctorium seeds and leaves, identifying high concentrations of [antioxidant](/ingredients/condition/antioxidant) polyphenols and demonstrating significant anticancer activity against cultured tumor cell lines in vitro. Duan et al. (2019) in the Journal of Pharmacology and Experimental Therapeutics (PMID 31467086) showed that aucubin isolated from indigo fruits protected mice against LPS-induced cardiac dysfunction by inhibiting NF-κB pathway activation, providing the first in vivo evidence of indigo fruit-derived cardioprotection. Wang YH (2020) in Zhongguo Zhong Yao Za Zhi (PMID 33496123) conducted a textual research review on medicinal blue herbs including Polygonum tinctorium, contextualizing the historical pharmacological use of indigo-producing plants in traditional Chinese medicine. Deng et al. (2013) in the British Journal of Dermatology (PMID 23909714) performed a systematic review and meta-analysis of plant extracts—including indigo-derived preparations—for topical management of psoriasis, finding preliminary evidence of [anti-inflammatory](/ingredients/condition/inflammation) efficacy in dermatological applications. ## Historical & Cultural Context - In Ayurvedic and Siddha systems, the Indigo plant (primarily leaves and roots) is traditionally used for wound healing, [anti-inflammatory](/ingredients/condition/inflammation) pastes, and liver-cleansing decoctions. However, the Indigo Berry fruit itself plays no significant role in canonical texts or traditional internal medicinal applications; crushed seeds have occasionally been used topically in some indigenous practices. ## Synergistic Combinations Role: Intention: Primary Pairings: ## Frequently Asked Questions ### What are the main health benefits of indigo berry? Indigo berry (Polygonum tinctorium fruit) provides antioxidant, anti-inflammatory, and cardioprotective benefits primarily attributed to aucubin and polyphenols. Duan et al. (2019, PMID 31467086) showed aucubin from indigo fruits protected against LPS-induced cardiac dysfunction in mice by inhibiting NF-κB signaling. Jang et al. (2012, PMID 22644642) further demonstrated potent antioxidant and anticancer properties from the plant's polyphenolic compounds. ### Is indigo berry the same as a blueberry or blue honeysuckle berry? No—indigo berry refers specifically to the fruit of Polygonum tinctorium (Japanese indigo), a plant in the Polygonaceae family historically cultivated for dye production and traditional medicine. It is botanically unrelated to blueberries (Vaccinium spp.) or blue honeysuckle berries (Lonicera caerulea), though all share anthocyanin pigments. The 'Indigo Blue Berries' cherry tomato (Solanum lycopersicum) is also a separate, unrelated cultivar. ### What is aucubin and why is it important in indigo berry? Aucubin is an iridoid glycoside and the principal bioactive compound isolated from Polygonum tinctorium fruits. Research by Duan et al. (2019, PMID 31467086) established that aucubin inhibits IκB kinase (IKK) phosphorylation, blocking NF-κB nuclear translocation and reducing pro-inflammatory cytokines such as TNF-α and IL-6. This mechanism underlies its documented cardioprotective and anti-inflammatory activity in preclinical models. ### Can indigo berry or indigo plant extracts help with skin conditions like psoriasis? Preliminary evidence suggests indigo-derived plant extracts may benefit inflammatory skin conditions. Deng et al. (2013, PMID 23909714) conducted a systematic review and meta-analysis in the British Journal of Dermatology evaluating plant extracts for topical psoriasis management, finding some support for indigo-related preparations. However, rigorous large-scale randomized controlled trials specifically on indigo berry for dermatological use remain lacking. ### Does indigo berry have anticancer properties? In vitro studies indicate anticancer potential. Jang et al. (2012, PMID 22644642) analyzed the chemical composition of Polygonum tinctorium seeds and leaves, identifying polyphenolic compounds with significant cytotoxic activity against cultured tumor cell lines. These results are promising but preliminary; no human clinical trials have yet evaluated indigo berry's anticancer efficacy, and in vitro findings do not directly translate to therapeutic outcomes in vivo. ### What forms of indigo berry are available for supplementation? Indigo berry is primarily available as seed extracts and concentrated phytochemical formulations rather than whole fruit supplements, since most research has focused on the bioactive compounds found in the seeds. Powder and liquid extract forms are the most common, as these concentrate the compounds believed to have antimicrobial and anti-inflammatory potential. Whole indigo berry as a fresh or dried consumable fruit is less commonly available in supplement markets compared to other berry types. ### Is there strong clinical evidence supporting indigo berry supplementation in humans? Current evidence for indigo berry is primarily derived from phytochemical analysis and in vitro studies rather than large-scale human clinical trials, limiting the strength of conclusions about its effectiveness. Most research has examined isolated compounds from indigo berry seeds in laboratory settings, which does not always translate to the same effects when consumed as a supplement. More rigorous human studies are needed to establish dosage recommendations and therapeutic benefits in real-world use. ### Are there any known safety concerns or contraindications with indigo berry supplements? Limited human safety data exists for indigo berry supplements due to minimal clinical research, making it difficult to establish comprehensive safety profiles for specific populations. As with any botanical extract, individuals with known allergies to berries or those taking medications should consult a healthcare provider before use. Pregnant, nursing, or pediatric populations should seek professional medical guidance before supplementing with indigo berry extract due to insufficient safety data in these groups. ## References Wang YH (2020). [Textual research on medicinal blue herbs]. Zhongguo Zhong Yao Za Zhi. PMID: 33496123 Deng S et al. (2013). Plant extracts for the topical management of psoriasis: a systematic review and meta-analysis. British Journal of Dermatology. PMID: 23909714 Jang HG et al. (2012). Chemical composition, antioxidant and anticancer effects of the seeds and leaves of indigo (Polygonum tinctorium Ait.) plant. Applied Biochemistry and Biotechnology. PMID: 22644642 Duan M et al. (2019). Indigo Fruits Ingredient, Aucubin, Protects against LPS-Induced Cardiac Dysfunction in Mice. Journal of Pharmacology and Experimental Therapeutics. PMID: 31467086 Ponder A et al. (2022). Polyphenols Content, Antioxidant Properties and Allergenic Potency of Organic and Conventional Blue Honeysuckle Berries. Molecules. PMID: 36144816 Karlsson T et al. (2022). Effect of the New Nordic Diet compared with usual care on glucose control in gestational diabetes mellitus: Study protocol for the randomized controlled trial intervention with new Nordic DIet in women with GestatiOnal diabetes mellitus (iNDIGO). Contemporary Clinical Trials. PMID: 35158086 Chen J et al. (2013). Posterior hyaloid detachment and internal limiting membrane peeling assisted by anthocyanins from acai fruit (Euterpe oleracea) and 10 other natural vital dyes: experimental study in cadaveric eyes. Retina. PMID: 22990318 Levin L et al. (2004). Evaluation of Argentinean white rot fungi for their ability to produce lignin-modifying enzymes and decolorize industrial dyes. Bioresource Technology. PMID: 15158509 --- *Source: Hermetica Superfoods Ingredient Encyclopedia — https://ingredients.hermeticasuperfoods.com* *License: CC BY-NC-SA 4.0 — Attribution required. Commercial use: admin@hermeticasuperfoods.com*